show Abstracthide AbstractUsing minor modifications to the AFLPseq protocol, we successfully recovered variable and useful markers that were applied to studies examining different scales of biological organization–from within populations to phylogenetic questions to the genus level and above. Three case studies on Acacia and Eucalyptus generated genomic data across the taxonomic hierarchies including—demonstrating the detection of Acacia pinguifolia individuals used in restoration and their population origins, regional phylogeography of Acacia pycnantha and SNP marker conservatism across some 70 million years of divergence amongst Myrtaceae.